ABSol – ein neues Produkt der Proteinnachweismethode
Die Vorteile auf einen Blick – das Amidoschwarz Protein Assay zur Optimierung von Proteinnachweismethoden!
- Buffer variation does not matter
TRIS and similar compounds can readily be present - Detergent content does not matter
SDS, Triton X-100, NP-40 do not interfere - Chaotropic salts do not matter
Urea and potassium iodide are no longer a problem! - Physical status of the protein does not matter
Native or denatured, dissolved, suspended or on NC, tissue slices, histological samples, homogenates or whole cells from culture … - Extremely wide range of applications
Chromatographic or cell culture monitoring, Western Blotting, .. - Minimal sample volume, high sensitivity
1 !l will do! – As little as 0.1 !g can readily be detected - Automatable without special equipment
Your good old photometer or microplate processor will do! - Easy to perform, no critical timing
Can be interrupted and left at any point - Background reading is unbeatably low
Can reduce background staining to 0.02 OD - Double determinations on the same sample are possible
Determine radio- or immunomarkers, metabolite content, … in the same sample!
Where can ABSol help you? ”Out here I need unlimitedly
stable equipment, yet a highly reliable method”
- Field work
Instant sample immobilization with minimal equipment, no cooling required, time span to follow-up is insignificant - Histological investigations
Determine the protein content of tissue sections or homogenates after other tests have been performed like metabolite quantitation or immuno-localization assays - Cell culture monitoring
Determine the protein content of whole cells, adherent, suspended or on microbeads - Column chromatography and routine tests
TRIS buffers and detergents do not interfere; Micro-assay requires 1 !l-samples only; reliable, sensitive and reproducible - 1- or 2D gel electrophoresis
Determine the protein content of single bands following transfer to nitrocellulose and immunologic or radioactivity analysis without worrying about interference by detergents and/or ampholytes