{"id":260,"date":"2019-10-22T13:36:51","date_gmt":"2019-10-22T11:36:51","guid":{"rendered":"http:\/\/dev.mos-technologies.de\/products\/amido-black-solution-protocol\/"},"modified":"2019-10-22T13:36:51","modified_gmt":"2019-10-22T11:36:51","slug":"amido-black-solution-protocol","status":"publish","type":"page","link":"https:\/\/mos-technologies.de\/en\/products\/amido-black-solution-protocol\/","title":{"rendered":"Amido Black Solution Protocol"},"content":{"rendered":"<div class=\"flex_column av_one_third  flex_column_div av-zero-column-padding first  avia-builder-el-0  el_before_av_heading  avia-builder-el-first  \" style='border-radius:0px; '><div  class='avia-image-container  av-styling-    avia-builder-el-1  avia-builder-el-no-sibling  avia-align-left '  itemprop=\"image\" itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/ImageObject\"  ><div class='avia-image-container-inner'><div class='avia-image-overlay-wrap'><img class='avia_image' src='https:\/\/mos-technologies.de\/wp-content\/uploads\/ABSol_Thumbnail.png' alt='' title='ABSol_Thumbnail' height=\"300\" width=\"300\"  itemprop=\"thumbnailUrl\"  \/><\/div><\/div><\/div><\/div><div  style='padding-bottom:10px; ' class='av-special-heading av-special-heading-h3    avia-builder-el-2  el_after_av_one_third  el_before_av_textblock  '><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >ABSol \u2013 the end to all your protein determination troubles<\/h3><div class='special-heading-border'><div class='special-heading-inner-border' ><\/div><\/div><\/div><\/p>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><p>Where can ABSol help you? \u201dOut here I need unlimitedly\u00a0stable equipment, yet a highly reliable method\u201d<\/p>\n<\/div><\/section>\n<div  style='padding-bottom:10px; ' class='av-special-heading av-special-heading-h3    avia-builder-el-4  el_after_av_textblock  el_before_av_textblock  '><h3 class='av-special-heading-tag '  itemprop=\"headline\"  >Determine radio- or immunomarkers, metabolite content, \u2026 in the same sample!<\/h3><div class='special-heading-border'><div class='special-heading-inner-border' ><\/div><\/div><\/div>\n<section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><div><\/div>\n<ol>\n<li>Buffer variation does not matter<br \/>\nTRIS and similar compounds can readily be present<\/li>\n<li>Detergent content does not matter<br \/>\nSDS, Triton X-100, NP-40 do not interfere<\/li>\n<li>Chaotropic salts do not matter<br \/>\nUrea and potassium iodide are no longer a problem!<\/li>\n<li>Physical status of the protein does not matter<br \/>\nNative or denatured, dissolved, suspended or on NC, tissue slices, histological samples, homogenates or whole cells from culture \u2026<\/li>\n<li>Extremely wide range of applications<br \/>\nChromatographic or cell culture monitoring, Western Blotting, ..<\/li>\n<li>Minimal sample volume, high sensitivity<br \/>\n1 !l will do! \u2013 As little as 0.1 !g can readily be detected<\/li>\n<li>Automatable without special equipment<br \/>\nYour good old photometer or microplate processor will do!<\/li>\n<li>Easy to perform, no critical timing<br \/>\nCan be interrupted and left at any point<\/li>\n<li>Background reading is unbeatably low<br \/>\nCan reduce background staining to 0.02 OD<\/li>\n<li>Double determinations on the same sample are possible<\/li>\n<\/ol>\n<p>Determine radio- or immunomarkers, metabolite content, \u2026 in the same sample!<br \/>\nWhere can ABSol help you? \u201dOut here I need unlimitedly<br \/>\nstable equipment, yet a highly reliable method\u201d<\/p>\n<ol>\n<li>Field work<br \/>\nInstant sample immobilization with minimal equipment, no cooling required, time span to follow-up is insignificant<\/li>\n<li>Histological investigations<br \/>\nDetermine the protein content of tissue sections or homogenates after other tests have been performed like metabolite quantitation or immuno-localization assays<\/li>\n<li>Cell culture monitoring<br \/>\nDetermine the protein content of whole cells, adherent, suspended or on microbeads<\/li>\n<li>Column chromatography and routine tests<br \/>\nTRIS buffers and detergents do not interfere; Micro-assay requires 1 !l-samples only; reliable, sensitive and reproducible<\/li>\n<li>1- or 2D gel electrophoresis<br \/>\nDetermine the protein content of single bands following transfer to nitrocellulose and immunologic or radioactivity analysis without worrying about interference by detergents and\/or ampholytes<\/li>\n<\/ol>\n<\/div><\/section>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"parent":222,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-260","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/pages\/260","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/comments?post=260"}],"version-history":[{"count":1,"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/pages\/260\/revisions"}],"predecessor-version":[{"id":261,"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/pages\/260\/revisions\/261"}],"up":[{"embeddable":true,"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/pages\/222"}],"wp:attachment":[{"href":"https:\/\/mos-technologies.de\/en\/wp-json\/wp\/v2\/media?parent=260"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}