ABSol – ein neues Produkt der Proteinnachweismethode

Seien Sie offen für scheinbar Unmögliches

Die Vorteile auf einen Blick – das Amidoschwarz Protein Assay zur Optimierung von Proteinnachweismethoden!

  1. Buffer variation does not matter
    TRIS and similar compounds can readily be present
  2. Detergent content does not matter
    SDS, Triton X-100, NP-40 do not interfere
  3. Chaotropic salts do not matter
    Urea and potassium iodide are no longer a problem!
  4. Physical status of the protein does not matter
    Native or denatured, dissolved, suspended or on NC, tissue slices, histological samples, homogenates or whole cells from culture …
  5. Extremely wide range of applications
    Chromatographic or cell culture monitoring, Western Blotting, ..
  6. Minimal sample volume, high sensitivity
    1 !l will do! – As little as 0.1 !g can readily be detected
  7. Automatable without special equipment
    Your good old photometer or microplate processor will do!
  8. Easy to perform, no critical timing
    Can be interrupted and left at any point
  9. Background reading is unbeatably low
    Can reduce background staining to 0.02 OD
  10. Double determinations on the same sample are possible

Determine radio- or immunomarkers, metabolite content, … in the same sample!
Where can ABSol help you? ”Out here I need unlimitedly
stable equipment, yet a highly reliable method”

  1. Field work
    Instant sample immobilization with minimal equipment, no cooling required, time span to follow-up is insignificant
  2. Histological investigations
    Determine the protein content of tissue sections or homogenates after other tests have been performed like metabolite quantitation or immuno-localization assays
  3. Cell culture monitoring
    Determine the protein content of whole cells, adherent, suspended or on microbeads
  4. Column chromatography and routine tests
    TRIS buffers and detergents do not interfere; Micro-assay requires 1 !l-samples only; reliable, sensitive and reproducible
  5. 1- or 2D gel electrophoresis
    Determine the protein content of single bands following transfer to nitrocellulose and immunologic or radioactivity analysis without worrying about interference by detergents and/or ampholytes